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禾苗专利菌种 解决农业种植难题丨一种复合微生物菌剂对茎线虫病害具有良好防效且可实现增产增收

  • 2026-06-04 00:36:03
禾苗专利菌种 解决农业种植难题丨一种复合微生物菌剂对茎线虫病害具有良好防效且可实现增产增收

说明摘要

      本发明涉及一种复合微生物菌剂,其包括贝莱斯芽孢杆菌HM-6和球孢白僵菌。所述贝莱斯芽孢杆菌HM-6的保藏编号为CGMCCNo.28606。本发明同时还涉及上述复合菌剂的制备方法和应用。本发明的复合微生物菌剂对茎线虫病害具有良好防效且可实现增产增收。贝莱斯芽孢杆菌(生防细菌)与球孢白僵菌(生防真菌)复合施用,可通过细菌抑菌+真菌寄生的协同作用,高效防控作物茎线虫病害,同时改良根际微环境、促进植株健壮。

This invention relates to a composite microbial inoculant, comprising Bacillus velezensis HM-6 and Beauveria bassiana. The preservation number of Bacillus velezensis HM-6 is CGMCC No.28606. The invention also covers the preparation method and application of the aforementioned composite inoculant. The composite microbial inoculant delivers excellent control efficacy against stem nematode diseases and can boost crop yield and economic benefits.The combined application of the biocontrol bacterium Bacillus velezensis and the biocontrol fungus Beauveria bassiana exerts synergistic effects via bacteriostatic action and fungal parasitism. It effectively prevents and controls crop stem nematode diseases, improves the rhizosphere microenvironment and promotes robust plant growth.

1.贝莱斯芽孢杆菌HM-6:抑菌+促抗双效

· 分泌脂肽类抗生素(伊枯草菌素、芬枯草菌素)与几丁质酶,直接破坏茎线虫体壁结构,抑制线虫卵孵化与幼虫活性,降低种群密度。

· 诱导作物产生系统抗性(SAR),激活植株体内防御酶(过氧化物酶、多酚氧化酶)活性,增强抗线虫侵染能力。

· 活化土壤中磷、钾及微量元素,促进根系发育,提升作物抗逆性,减少线虫侵染后的发病程度。

2.球孢白僵菌:寄生致死+持续控害

· 孢子接触茎线虫体壁后萌发,产生侵染钉穿透线虫体壁,在体内繁殖并分泌白僵菌素,掠夺线虫营养使其致死。

· 线虫死亡后,菌丝体在其体内形成新的孢子,随土壤水分扩散,实现对茎线虫的持续防控,延长防效周期。

3.协同增效优势

· 贝莱斯芽孢杆菌为球孢白僵菌提供适宜的根际微环境(调节pH、分泌营养物质),提升白僵菌孢子萌发率;白僵菌的寄生作用可降低线虫对细菌的抗性,二者复合防效比单菌提升30%-50%。

1. Bacillus velezensis HM-6: Dual effects of bacteriostasis and stress resistance enhancement

· It secretes lipopeptide antibiotics including iturin and fengycin, as well as chitinase. These substances directly damage the body wall structure of stem nematodes, inhibit nematode egg hatching and larval activity, and reduce the population density.

· It induces systemic acquired resistance (SAR) in crops, activates the activity of defensive enzymes such as peroxidase and polyphenol oxidase in plants, and strengthens the capacity to resist nematode infestation.

· It activates phosphorus, potassium and trace elements in soil, facilitates root development, improves crop stress tolerance, and alleviates disease severity caused by nematode infection.

2. Beauveria bassiana: Parasitic lethality and sustained pest control

· After spores attach to the body wall of stem nematodes, they germinate and generate infection pegs to penetrate the body wall. The fungus proliferates inside the nematode and secretes beauvericin, seizing nutrients and leading to nematode death.

· New spores form inside dead nematodes. Dispersed along with soil moisture, they realize long-term suppression against stem nematodes and extend the effective control period.

3. Synergistic advantages

· Bacillus velezensis creates a favorable rhizosphere microenvironment by adjusting pH value and secreting nutrients, which improves the germination rate of Beauveria bassiana spores. The parasitic effect of Beauveria bassiana reduces nematode resistance to bacteria. The combined control efficacy of the two strains is 30%-50% higher than that of single strain application.

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  1. CONNECT:[ UseTime:0.000888s ] mysql:host=127.0.0.1;port=3306;dbname=h_mffb;charset=utf8mb4
  2. SHOW FULL COLUMNS FROM `fenlei` [ RunTime:0.001647s ]
  3. SELECT * FROM `fenlei` WHERE `fid` = 0 [ RunTime:0.000722s ]
  4. SELECT * FROM `fenlei` WHERE `fid` = 63 [ RunTime:0.000668s ]
  5. SHOW FULL COLUMNS FROM `set` [ RunTime:0.001309s ]
  6. SELECT * FROM `set` [ RunTime:0.000607s ]
  7. SHOW FULL COLUMNS FROM `article` [ RunTime:0.001506s ]
  8. SELECT * FROM `article` WHERE `id` = 539977 LIMIT 1 [ RunTime:0.000948s ]
  9. UPDATE `article` SET `lasttime` = 1780595716 WHERE `id` = 539977 [ RunTime:0.015941s ]
  10. SELECT * FROM `fenlei` WHERE `id` = 64 LIMIT 1 [ RunTime:0.000573s ]
  11. SELECT * FROM `article` WHERE `id` < 539977 ORDER BY `id` DESC LIMIT 1 [ RunTime:0.001187s ]
  12. SELECT * FROM `article` WHERE `id` > 539977 ORDER BY `id` ASC LIMIT 1 [ RunTime:0.001114s ]
  13. SELECT * FROM `article` WHERE `id` < 539977 ORDER BY `id` DESC LIMIT 10 [ RunTime:0.006916s ]
  14. SELECT * FROM `article` WHERE `id` < 539977 ORDER BY `id` DESC LIMIT 10,10 [ RunTime:0.010979s ]
  15. SELECT * FROM `article` WHERE `id` < 539977 ORDER BY `id` DESC LIMIT 20,10 [ RunTime:0.034393s ]
0.177601s