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青岛农业大学-免疫调节六肽

  • 2026-05-09 03:08:38
青岛农业大学-免疫调节六肽
1.专利号：CN121574195A，专利名称：一一种从法氏囊中分离的六肽及其在免疫调节中的应用，申请日：2025.12.05，公布（开）日：2026.02.27，申请人：青岛农业大学
本发明的目的在于提供一种具有免疫调节作用的从法氏囊中分离的六肽，其氨基酸序列为RNKDVY。本发 明是从法氏囊提取物中分离法得到的，法氏囊六 肽结构简单，无毒副作用，免疫原性极弱。对抗体 产生也具有诱导作用，同时可调节细胞因子的产 生，淋巴细胞的增殖，促进细胞免疫反应。本发明 既可从法氏囊中分离提取，也可化学合成，成本 低，可大量生产。此外，还以剂量依赖性方式调节B细胞的分化，具有广泛的应用前景，如在免疫调节和治疗等方面等应用。
1.1权利要求保护范围
1.2相关试验测试
1.2.1BP‑E1作为免疫增强剂的应用
分析结果表明1mg/Kg法氏囊四肽（BP‑E1）在一免时能显著调 节抗体IgG的水平，二免时1mg/Kg能显著调节抗体IgG的水平（图2、图3）。
1.2.2：一免血清抗体效果表
在小鼠实验中，1mg/Kg 多肽实验组IL‑4、IFN‑g均显著高于疫苗对照组（图4、5）。表明1mg/Kg法氏囊六肽不仅能提 高代表Th1类型免疫反应得细胞因子IFN‑g的水平，同时能显著提高代表Th2类型的细胞因 子IL‑4的水平。
1.2.3、脾细胞增殖试验
小鼠二免后1周，脱颈处死小鼠，取出脾脏，制备得到脾淋巴细胞悬液，将脾淋巴细 胞悬液分装到96孔细胞板上（107个细胞/孔），加入1μg/mL AIV抗原刺激细胞，每孔100μL， 并设置只加入完全培养基调零孔。于5 ％CO2 细胞培养箱中37 ℃培养48 h后，每孔加入10μ L CCK8试剂。细胞培养箱37 ℃孵育4h后，于450 nm测定OD值，分析结果。结果，1mg/K的BP E1极其显著增加脾脏淋巴细胞的活性，见图6。
1.2.4：DT‑40细胞增值试验
DT40细胞其有与鸡法氏囊前 B淋巴细胞相同的生物学特性，能为研究法氏囊活性 肽对B细胞的作用机制提供理想的细胞模型。本研究以 DT40细胞为禽前B细胞模型，使用法氏囊活性肽RNKDVY （SEQ ID NO:1）分别以 100ug/ml、 75ug/ml、50ug/ml、25ug/mg的浓度 刺激DT40 细胞，刺激24小时后，加入10 ml/孔 CCK‑8，孵育2小时，OD450 nm读值，分析结 果。结果，与PBS对照组比较100ug/ml BP‑E1能显著增加DT‑40细胞的活性，100ug/ml BP‑E1 对DT‑40细胞的活性增加极其显著（图7）。
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  1. CONNECT:[ UseTime:0.000717s ] mysql:host=127.0.0.1;port=3306;dbname=h_mffb;charset=utf8mb4
  2. SHOW FULL COLUMNS FROM `fenlei` [ RunTime:0.001302s ]
  3. SELECT * FROM `fenlei` WHERE `fid` = 0 [ RunTime:0.000537s ]
  4. SELECT * FROM `fenlei` WHERE `fid` = 63 [ RunTime:0.000633s ]
  5. SHOW FULL COLUMNS FROM `set` [ RunTime:0.001071s ]
  6. SELECT * FROM `set` [ RunTime:0.002160s ]
  7. SHOW FULL COLUMNS FROM `article` [ RunTime:0.001562s ]
  8. SELECT * FROM `article` WHERE `id` = 503006 LIMIT 1 [ RunTime:0.007399s ]
  9. UPDATE `article` SET `lasttime` = 1778449595 WHERE `id` = 503006 [ RunTime:0.010925s ]
  10. SELECT * FROM `fenlei` WHERE `id` = 64 LIMIT 1 [ RunTime:0.001041s ]
  11. SELECT * FROM `article` WHERE `id` < 503006 ORDER BY `id` DESC LIMIT 1 [ RunTime:0.013906s ]
  12. SELECT * FROM `article` WHERE `id` > 503006 ORDER BY `id` ASC LIMIT 1 [ RunTime:0.015172s ]
  13. SELECT * FROM `article` WHERE `id` < 503006 ORDER BY `id` DESC LIMIT 10 [ RunTime:0.056468s ]
  14. SELECT * FROM `article` WHERE `id` < 503006 ORDER BY `id` DESC LIMIT 10,10 [ RunTime:0.069855s ]
  15. SELECT * FROM `article` WHERE `id` < 503006 ORDER BY `id` DESC LIMIT 20,10 [ RunTime:0.084027s ]
0.401117s